Inward Ca²⁺ current through the polycystin-2-dependent channels of renal primary cilia

Abstract

In 15% of cases, autosomal dominant polycystic kidney disease arises from defects in polycystin-2 (PC2). PC2 is a member of the polycystin transient receptor potential subfamily of cation-conducting channels and is expressed in the endoplasmic reticulum and primary cilium of renal epithelial cells. PC2 opposes a procystogenic influence of the cilium, and it has been proposed that this beneficial effect is mediated in part by a flow of Ca²⁺ through PC2 channels into the primary cilium. However, previous efforts to determine the permeability of PC2 channels to Ca²⁺ have yielded widely varying results. Here, we report the mean macroscopic Ca²⁺ influx through native PC2 channels in the primary cilia of mIMCD-3 cells, which are derived from the murine inner medullary collecting duct. Under conditions designed to isolate inward Ca²⁺ currents, a small inward Ca²⁺ current was detected in cilia with active PC2 channels but not in cilia lacking those channels. The current was activated by the addition of 10 µM internal Ca²⁺, which is known to activate ciliary PC2 channels. It was blocked by 10 µM isosakuranetin, which blocks the same channels. On average, the current amplitude was -1.8 pA at -190 mV; its conductance from -50 to -200 mV averaged 20 pS. Thus, native PC2 channels of renal primary cilia are able to conduct a small but detectable Ca²⁺ influx under the conditions tested. The possible consequences of this influx are discussed.<b>NEW & NOTEWORTHY</b> In autosomal dominant polycystic kidney disease, it is proposed that Ca²⁺ entering the primary cilium through polycystin-2 (PC2) channels may limit the formation of cysts. Recent studies predict that any macroscopic Ca²⁺ influx through these channels should be small. We report that the native PC2 channels in primary cilia of cultured renal epithelial cells can allow a small macroscopic calcium influx. This may allow a significant accumulation of Ca²⁺ in the cilium in vivo.