Stomatal conductance of tomato leaves is regulated by both abscisic acid and leaf water potential under combined water and salt stress

Abstract

Stomatal conductance (g_s ) affects water use efficiency (WUE) through coordinating photosynthesis and transpiration and is regulated by chemical and/or hydraulic signals. However, the regulation mechanism of g_s of tomato leaves has not been fully explored under combined water and salt stress. Here, we set up four salt treatments and two water treatments in a climate greenhouse and measured stomatal morphologies and conductance and other photosynthesis parameters. Water and salt stress reduced stomatal length (SL), width, perimeter, area (a_max ), density (SD), and the maximum stomatal conductance (g_smax ). Water and salt stress had a separate weakening effect on net photosynthetic rate (A) and transpiration rate but interactively reduced g_s . The contents of abscisic acid (ABA) and Na⁺ in tomato leaves increased with the NaCl concentration, while leaf water potential (Ψ_l ) and chlorophyll content decreased. Under full irrigation, g_smax was coordinated by SD and a_max , and g_s by ABA content under salt stress. Under water and salt combined stress, g_smax was affected by a_max , and g_s was coordinated with ABA and Ψ_l . The decrease of A was caused by both a reduction of chlorophyll content and g_s under water and salt stress. Intrinsic WUE did not reduce under full irrigation or mild to moderate salt stress but decreased under a combination of water and severe salt stress, indicating that the leaves of the tested tomato cultivar performed better under moderate salt stress. Collectively, these results can provide useful insights for the efficient management of water and salt to adapt to drought and high salt environments.