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The polysaccharide isolated from Pleurotus nebrodensis （PN-S） shows immune-stimulating activity in RAW264.7 macrophages
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2015
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Immune-stimulating ActivityInflammatory Lung DiseaseLung InflammationNitric OxideRaw264.7 CellsInnate Immune SystemImmunologyImmune RegulationPathologyInnate ImmunityImmune SystemInflammationRaw264.7 MacrophagesMacrophage BiologyChronic InflammationImmune SurveillanceImmune FunctionInflammatory DiseasePhagocyteCytokineImmune Cell DevelopmentInflammation BiologyRaw264.7 Macrophages.pn-sPleurotus Nebrodensis （Pn-s）Medicine
A novel Pleurotus nebrodensis polysaccharide(PN-S) was purified andcharacterized,and its immune-stimulating activity was evaluated in RAW264.7 macrophages.PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner,as determined by the MTT assay.After exposure to PN-S,the phagocytosis of the macrophages was significantly improved,with remarkable changes in morphology being observed.Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases.PN-S treatment enhanced the productions of interleukin-6(IL-6),nitric oxide(NO),interferon gamma(INF-γ),and tumor necrosis factor-α(TNF-α) in the macrophages,with up-regulation of m RNA expressions of interleukin-6(IL-6),inducible nitric oxide synthase(i NOS),interferon gamma(INF-γ) and tumor necrosis factor-α(TNF-α) being observed in a dose-dependent manner,as measured by q RT-PCR.In conclusion,these results suggest that the purified PN-S can improve immunity by activating macrophages.