mRNALocater: Enhance the prediction accuracy of eukaryotic mRNA subcellular localization by using model fusion strategy

Abstract

The functions of mRNAs are closely correlated with their locations in cells. Knowledge about the subcellular locations of mRNA is helpful to understand their biological functions. In recent years, it has become a hot topic to develop effective computational models to predict eukaryotic mRNA subcellular localizations. However, existing state-of-the-art models still have certain deficiencies in terms of prediction accuracy and generalization ability. Therefore, it is urgent to develop novel methods to accurately predict mRNA subcellular localizations. In this study, a novel method called mRNALocater was proposed to detect the subcellular localization of eukaryotic mRNA by adopting the model fusion strategy. To fully extract information from mRNA sequences, the electron-ion interaction pseudopotential and pseudo k-tuple nucleotide composition were used to encode the sequences. Moreover, the correlation coefficient filtering algorithm and feature forward search technology were used to mine hidden feature information, which guarantees that mRNALocater can be more effectively applied to new sequences. The results based on the independent dataset tests demonstrate that mRNALocater yields promising performances for predicting eukaryotic mRNA subcellular localizations and is a powerful tool in practical applications. A freely available online web server for mRNALocater has been established at http://bio-bigdata.cn/mRNALocater. The functions of mRNAs are closely correlated with their locations in cells. Knowledge about the subcellular locations of mRNA is helpful to understand their biological functions. In recent years, it has become a hot topic to develop effective computational models to predict eukaryotic mRNA subcellular localizations. However, existing state-of-the-art models still have certain deficiencies in terms of prediction accuracy and generalization ability. Therefore, it is urgent to develop novel methods to accurately predict mRNA subcellular localizations. In this study, a novel method called mRNALocater was proposed to detect the subcellular localization of eukaryotic mRNA by adopting the model fusion strategy. To fully extract information from mRNA sequences, the electron-ion interaction pseudopotential and pseudo k-tuple nucleotide composition were used to encode the sequences. Moreover, the correlation coefficient filtering algorithm and feature forward search technology were used to mine hidden feature information, which guarantees that mRNALocater can be more effectively applied to new sequences. The results based on the independent dataset tests demonstrate that mRNALocater yields promising performances for predicting eukaryotic mRNA subcellular localizations and is a powerful tool in practical applications. A freely available online web server for mRNALocater has been established at http://bio-bigdata.cn/mRNALocater.