Humana Press eBooks · 2003 · 151 citations · 0 references
Life SciencesBiologyDevelopmental BiologyAssisted Reproductive TechnologiesEmbryologyIn Vitro FertilizationEarly Embryonic DevelopmentEmbryo BiotechnologyEmbryo DevelopmentEmbryo ManipulationEmbryonic DevelopmentTransgenesis StrategiesAmphibian EmbryologyEarly Vertebrate DevelopmentEmbryo CreationEmbryonic Gene TransferTransgenic Model Organism DevelopmentXenopus Developmental Models
The early amphibian embryo has been widely used as a model organism for studying early vertebrate development. This chapter describes in detail a new and very efficient method for generating transgenic Xenopus embryos. At the end of the chapter, a new method for fertilizing in vitro matured oocytes is also introduced.