Peripheral Blood from Rheumatoid Arthritis Patients Shows Decreased Treg CD25 Expression and Reduced Frequency of Effector Treg Subpopulation

Abstract

Rheumatoid arthritis (RA) is a common autoimmune disease characterized by immune cell infiltration of the synovium, leading to the loss of cartilage, bone, and joint function. Although regulatory T (T_reg) cells are thought to modulate the initiation and progression of RA, a consensus has yet to be reached regarding the function and composition of T_reg cells in RA patients. To address these discrepancies, we analyzed not only the total T_reg frequency but also that of T_reg subpopulations in the peripheral blood of RA patients and healthy controls by flow cytometry. We found that the total T_reg population was not significantly different between RA and control subjects. However, the effector T_reg cell subgroup, defined as CD45RA^-CD25^hi, showed markedly decreased frequency in RA patients. In addition, the total T_reg population from RA patients showed a significant decline in the expression of CD25. Both the naïve and effector T_reg subgroups also showed marked reduction of CD25 expression in RA patients compared to controls. These data suggest that the decreased frequency of effector T_reg cells and overall reduction of CD25 expression in T_reg cells in the peripheral blood may be evidence of altered T_reg homeostasis associated with RA pathogenesis.