ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS OF BANGUN-BANGUN (Coleus amboinicus, L.) LEAVES FROM NORTH SUMATERA, INDONESIA

Abstract

Coleus amboinicus, Lour has been successfully isolated and characterized active isolates from the ethyl acetate fraction and tested its antioxidant activity. Isolation was carried out by maceration method with methanol followed by stratified fractionation with n-hexane, chlorofrom and ethyl acetate. The each fraction was tested for antioxidant potential by DPPH method at a wavelength of 515 nm and the ethyl acetate fraction gave a better antioxidant potential then it's purified. Purification using by gravitational column chromatography with the stationary phase silica gel 60 and the mobile phase ratio of n-hexane: ethyl acetate with polarity are gradually increased. Isolates were characterized by UV, FT-IR, NMR, and GC-MS spectrophotometry. Antioxidant activity (IC50) test obtained 386.46. The molecular formula is C22H28O6 isolate with the name of the chemical compound 16-hydroxy-7-acetoxyroyleanone (syn. 16-hydroxy-7-O-acetylhorminone).