High-sensitivity detection of Concanavalin A using MoS ₂ -based field effect transistor biosensor

Abstract

Abstract The specific interaction between sugar and lectin plays a key role in various human diseases and projects remarkable significance to the development of a lectin biosensor for diagnosis and drug discovery. An inorganic nano MoS 2 field-effect transistor (FET)-based D-Mannose biosensor was developed in this paper using a simple assembly process. First, gold nanoparticles were functionalized on the surface of MoS 2 . Then, the β -mercaptoethylamine was self-assembled on the gold surface via Au–S bonds to derivatize amino groups. Finally, the probe molecules D-Mannose for biological protein were immobilized on the FET via the Schiff base reaction. Results from scanning electron microscope and x-ray photoelectron spectroscopy analysis confirmed the entire assembly procedure and the feasible interaction of the FET sensor with Concanavalin A (ConA). The fabricated FET biosensors can reliably detect the target ConA with a detection limit below 105 nm. The current study shows the potential of MoS 2 FET biosensors for ConA specific detection and clarifies the design of biosensors for the early diagnosis of glycomics and drug screening in medicine.