Journal of Toxicologic Pathology · 2021 · 31 citations · 1 references
MedicinePathologyHistologyHistopathologyImmunotechnologyAntibody CharacterizationAntibody FunctionEnzyme-linked Immunosorbent AssayImmunohistochemistryAntibodiesExperimental HistopathologyHistopathological EvaluationImmunohistochemical MarkersImmunohistochemical StainingImmunohistochemistry AssaysPrimary AntibodiesReactivity
Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.). With the aim of sharing information about the technical aspects of immunohistochemistry and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during 2018–2019 among members of the Conference on Experimental Animal Histopathology. The report details IHC staining conditions, including fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time and temperature, positive control tissue, blocking condition, and secondary antibody information. The survey identified 509 primary antibodies across 220 types, updating the 2017 CEAH report.
With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017.
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