Profiling of RNA N6-Methyladenosine Methylation Reveals the Critical Role of m6A in Chicken Adipose Deposition

Abstract

One of the main objectives of broiler breeding is to prevent excessive abdominal adipose deposition. The role of RNA modification in adipose deposition is not clear. This study was aimed to map m⁶A modification landscape in chicken adipose tissue. MeRIP-seq was performed to compare the differences in m⁶A methylation pattern between fat and lean broilers. We found that start codons, stop codons, coding regions, and 3'-untranslated regions were generally enriched for m⁶A peaks. The high m⁶A methylated genes (fat birds vs. lean birds) were primarily associated with fatty acid biosynthesis and fatty acid metabolism, while the low m⁶A methylated genes were mainly involved in processes associated with development. Furthermore, we found that the mRNA levels of many genes may be regulated by m⁶A modification. This is the first comprehensive characterization of m⁶A patterns in the chicken adipose transcriptome, and provides a basis for studying the role of m⁶A modification in fat deposition.