Ozonated Aloe vera Oil Effective Increased the Number of Fibroblasts and Collagen Thickening in the Healing Response of Full-Thickness Skin Defects

Abstract

Objective. This study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts. Methods. This was an experimental research method using control groups and treatment groups with a posttest only control group design. The results showed that collagen thickness in wounds tended to increase, assessed on day 3 and day 7 using Masson’s trichrome staining and microscopic evaluation. Results. There was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal–Wallis test, with a value of <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>p</a:mi> <a:mo>=</a:mo> <a:mn>0.001</a:mn> </a:math> <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"> <c:mfenced open="(" close=")" separators="|"> <c:mrow> <c:mi>p</c:mi> <c:mo>&lt;</c:mo> <c:mn>0.05</c:mn> </c:mrow> </c:mfenced> </c:math> , resulting in a significant difference in wound size reduction between the groups. Further post hoc analysis using the Mann–Whitney test indicated a significant difference between the control groups and the treatment groups (P0, P1 versus P3, P4, P5, P8, P9, and P10) with a value of <h:math xmlns:h="http://www.w3.org/1998/Math/MathML" id="M3"> <h:mi>p</h:mi> <h:mo>=</h:mo> <h:mn>0.009</h:mn> </h:math> <j:math xmlns:j="http://www.w3.org/1998/Math/MathML" id="M4"> <j:mfenced open="(" close=")" separators="|"> <j:mrow> <j:mi>p</j:mi> <j:mo>&lt;</j:mo> <j:mn>0.05</j:mn> </j:mrow> </j:mfenced> </j:math> . Conclusions. Ozonated Aloe vera oil is effective in increasing the healing response of full-thickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats.