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Low-temperature incubation improves both knock-in and knock-down efficiencies by the CRISPR/Cas9 system in Xenopus laevis as revealed by quantitative analysis

Xenopus LaevisDevelopmental BiologyCrispr/cas9 SystemGeneticsGenetic EngineeringMolecular GeneticsGene EditingGenome EditingLow-temperature IncubationGene ExpressionMedicineOff-target EffectCrispr