Upconversion Nanoparticle Decorated Spider Silks as Single-Cell Thermometers

Abstract

Noninvasive and sensitive thermometry of a single living cell is crucial to the analysis of fundamental cellular processes and applications to cancer diagnosis. Optical fibers decorated with temperature-sensitive nanomaterials have become widely used instruments for biosensing temperature. However, current silica fibers exhibit low compatibility and degradability in biosystems. In this work, we employ spider silks as natural optical fibers to construct biocompatible thermometers. The spider silks were drawn directly from <i>Araneus ventricosus</i> and were decorated with core-shell upconversion nanoparticles (UCNPs) via a photophoretic effect. By measuring the fluorescence spectra of the UCNPs on the spider silks, the membrane temperature of a single breast cancer cell was obtained with absolute and relative sensitivities ranging from 3.3 to 4.5 × 10^-3 K^-1 and 0.2 to 0.8% K^-1, respectively. Additionally, the temperature variation during apoptosis was monitored by the thermometer in real time. This work provides a biocompatible tool for precise biosensing and single-cell analysis.