2002 · 27 citations · 5 references
ComputingComputer ScienceInformation TechnologyInformation RetrievalKnowledge RepresentationTerminology StudiesConcept ExtractionInformation ExtractionKnowledge ExtractionOntology EngineeringTerminology ManagementTerminology ResourcesConceptual ContentConceptual RelationsOntologiesOntology-based Information RetrievalOntology-based RetrievalTerminological Database ConstructionTerminological OntologyTerminology Resource Integration
<b>Aim:</b> To develop and validate a simple method using LC-MS/MS for determination of dextromethorphan (DXM) and dextrorphan (DT) in human oral fluid. <b>Results:</b> Following protein precipitation, chromatographic separation used a phenyl column with isocratic elution (1 ml/min) of 10 mM ammonium-formate buffer and acetonitrile (65:35; v/v) with 0.1% formic acid. Retention times were 2.6 min for DT and 5 min for DXM. Total run time was 7 min. The intra- and inter-assay deviations (accuracy) for DT (1-100 ng/ml) and DXM (5-1000 ng/ml) ranged from -13.6 to 8.8% and -9.6 to 5.7%, respectively. Precision variations were ≤7.5%. Matrix effect was ≤11.8%. <b>Conclusion:</b> This method may prove helpful for quantification of DT and DXM in oral fluid for either clinical or toxicological purposes.
5