Roseomonas coralli sp. nov., a heavy metal resistant bacterium isolated from coral

Abstract

A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104 T , was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province, PR China. Colonies of the strain were non-motile cocci and pink. The strain grew at 15–34 °C (optimum, 28 °C), pH 4.5–8.0 (optimum, pH 7.0) and with 0–4% (w/v) NaCl (optimum, 0–2 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0104 T was closely related to Roseomonas deserti JCM 31275 T (96.2 %), Roseomonas vastitatis KCTC 62043 T (96.0 %), Roseomonas aerofrigidensis JCM 31878 T (95.9 %) and Roseomonas oryzae KCTC 42542 T (95.7 %). The strain had an assembly size of 5.0 Mb and a G+C content of 71.0mol%. Genes involved in copper, cadmium, lead, arsenic and zinc resistance were identified in the genome of strain M0104 T . The digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain M0104 T and those of closely related type strains were 19.4–24.9 % and 74.3–81.8 %, respectively. Strain M0104 T contained C 18:1 ω 7 c , C 18:3 ω 3 c , anteiso C 11:0 and C 16:0 as the major fatty acids (>7 %) and ubiquinone Q-10 as the sole isoprenoid quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine were its major polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic properties, strain M0104 T is proposed to represent a novel species within the genus Roseomonas , for which the name Roseomonas coralli sp. nov. is proposed. The type strain is M0104 T (=KCTC 62359 T =MCCC 1K03632 T ).