A colorimetric sensor array for the classification of biologically relevant tri-, di- and mono-phosphates

Abstract

We describe here a supramolecular sensing system for the array-based classification of biologically relevant mono-, di-, and tri-phosphates in aqueous buffer. The system is based on a colorimetric indicator displacement assay (IDA) with six sensor elements. Each sensor element is comprised of an equimolar cyclic peptide-colorimetric indicator ensemble. Correct classification of the eleven analytes into mono-, di-, tri-, or pyrophosphate was achieved through both principal component analysis (PCA) and linear discriminant analysis (LDA).