Integrated Elastomer Fluidic Lab-on-a-chip Surface Patterning and DNA Diagnostics

Abstract

We recently developed a method of multilayer fabrication for elastomeric devices, which we used to fabricate monolithic active valves and pumps. Here we describe efforts to use these pumps and valves in an integrated DNA diagnostic chip and show results of a key component, surface patterning, with two different kind of surface chemistries by using similar elastomeric channel devices. Flow control, reagent metering, in-line mixing and loop circulations are also demonstrated.