PubMed · 1990 · 13 citations · 0 references
Veterinary MedicineImmunologyInfectious DiseasesDiagnostic ParasitologyHelminthologyParasitologyAntigen Detection AssaysClinical ParasitologyHelminth DiagnosticsMedical ParasitologyParasitic Disease DiagnosisParasitic Infestation DetectionAdult Worm AntigensAssay SpecificityDiagnostics Of Parasitic DiseasesHemagglutination AssaysImmunoassay Cross-reactivityImmunodiagnostic AssaysParasitic DetectionSerological Diagnosis Of Parasitic Infections
Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination tests (IHA) were evaluated for serodiagnosis of human paragonimiasis caused by Paragonimus heterotremus using homologous adult worm extract as antigen. IgG-ELISA was the most sensitive, being positive in all paragonimiasis sera tested while IHA and IgA-ELISA gave 88% and 59% positive rates respectively. Cross reactivity in IgG-ELISA was detected with fascioliasis sera, producing overall assay specificity of 97%. It is suggested that IgG-ELISA is a reliable serodiagnostic test for human paragonimiasis caused by Paragonimus heterotremus.