A promising technology for wound healing; <i>in-vitro</i> and <i>in-vivo</i> evaluation of chitosan nano-biocomposite films containing gentamicin

Abstract

<b>Aim:</b> This paper aims to study <i>in-vitro</i> and <i>in-vivo</i> evaluation of chitosan (CHI) biocomposite of gentamicin nanoparticles (GNPs) for wound healing. <b>Methods:</b> In this study, CHI nanoparticles (NPs) were prepared using the ionic gelation technique. GNP biocomposites were examined on the excision wound model in Wistar rats to determine the <i>in-vivo</i> efficiency. <b>Results:</b> The diameter and zeta potential of NPs were between 151-212.9 nm and 37.2 - 51.1 mV, respectively. The entrapment efficiency was in an acceptable range of 36.6-42.7% w/w. The release test information was fitted to mathematical models (Zero, First order, Higuchi, and Korsmeyer-Peppas), and according to calculations, the kinetics of drug release followed the Korsmeyer-Peppas model. A comparison of thermograms revealed that the drug was present in the formulation in a non-crystalline form. <b>Conclusion:</b> Histological studies of the wound showed that the rate of skin tissue repair was higher in the GNP biocomposite treatment group than in the others.