Illuminating type collections of nectriaceous fungi in Saccardo's fungarium

Abstract

Specimens of <i>Nectria</i> spp. and <i>Nectriella rufofusca</i> were obtained from the fungarium of Pier Andrea Saccardo, and investigated via a morphological and molecular approach based on MiSeq technology. ITS1 and ITS2 sequences were successfully obtained from 24 specimens identified as '<i>Nectria</i>' sensu Saccardo (including 20 types) and from the type specimen of <i>Nectriella rufofusca</i>. For <i>Nectria ambigua</i>, <i>N</i>. <i>radians</i> and <i>N. tjibodensis</i> only the ITS1 sequence was recovered. On the basis of morphological and molecular analyses new nomenclatural combinations for <i>Nectria albofimbriata</i>, <i>N. ambigua</i>, <i>N. ambigua</i> var. <i>pallens</i>, <i>N. granuligera</i>, <i>N. peziza</i> subsp. <i>reyesiana</i>, <i>N. radians</i>, <i>N. squamuligera</i>, <i>N. tjibodensis</i> and new synonymies for <i>N. congesta</i>, <i>N. flageoletiana</i>, <i>N.</i> <i>phyllostachydis</i>, <i>N. sordescens</i> and <i>N. tjibodensis</i> var. <i>crebrior</i> are proposed. Furthermore, the current classification is confirmed for <i>Nectria coronata</i>, <i>N. cyanostoma</i>, <i>N. dolichospora</i>, <i>N. illudens</i>, <i>N. leucotricha</i>, <i>N. mantuana</i>, <i>N. raripila</i> and <i>Nectriella rufofusca</i>. This is the first time that these more than 100-yr-old specimens are subjected to molecular analysis, thereby providing important new DNA sequence data authentic for these names.