A Multidrug-Resistant <i>Salmonella</i> Infantis Clone is Spreading and Recombining in the United States

Abstract

Recently, there have been reports worldwide of a multidrug-resistant, emergent <i>Salmonella</i> Infantis (ESI) clone with a large megaplasmid (pESI), often containing the extended-spectrum beta-lactamase gene <i>bla</i>_CTX-M-65. This clone also has a <i>gyrA</i> mutation conferring fluoroquinolone resistance, further limiting treatment options. In the United States, this clone has also been found in poultry sources, indicating a likely source of human illnesses. We conducted short-read sequencing of <i>Salmonella enterica</i> isolated from retail meats as part of routine surveillance by the National Antimicrobial Resistance Monitoring System (NARMS). We analyzed the resulting data temporally and geographically to determine when and where the ESI clone has spread in the United States. We found the ESI clone was first found in retail meats in Tennessee in 2014, but by 2019 was throughout the United States and comprised 29% of all <i>Salmonella</i> isolated from retail chickens, and 7% from retail turkey. Of these isolates, 85.0% were within 20 single nucleotide polymorphisms (SNPs) of those causing human illnesses. Long-read sequencing data indicated substantial recombination in the pESI plasmid resulting in the presence of 0-10 resistance genes, despite all their chromosomes being within 31 SNPs of one another. This work demonstrates the rapid spread of this clone of <i>Salmonella</i> Infantis in poultry in the United States, with the potential for increased burden of human illness attributed to this multidrug-resistant pathogen.