ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms

Abstract

Exocytic membrane traffic can be visualized using regulatable fluorescent secretory cargoes. In cells from diverse eukaryotes, a red fluorescent fusion protein termed ESCargo can be trapped as aggregates in the endoplasmic reticulum and then released by ligand addition to create a wave of transport through the secretory pathway. This tool should have broad utility.