Novel Antiviral and Antibacterial Activities of Hibiscus schizopetalus

Abstract

<i>Hibiscus schizopetalus</i> (Dyer) Hook.f. (Malvaceae) is an ornamental plant. The aim was to investigate its antimicrobial and antioxidant activities. In vitro antiviral, antibacterial, and antioxidant activities of the 70% ethanolic extract (Et-E) of the aerial parts of the plant were determined. The Dichloromethane Fraction (DCM-F) and the <i>n</i>-Butanol Fraction (Bu-F) were assessed using Liquid chromatography-mass spectrometry (LC-MS). The DCM-F showed higher antiviral activities against Coxsackie B4 (CoxB4) viruses (IC₅₀ = 64.13 µg/mL) and adenoviruses (IC₅₀ = 54.88 µg/mL) than acyclovir (IC₅₀ = 72.79 µg/mL for CoxB4 viruses; IC₅₀ = 91.92 µg/mL for adenoviruses). The DCM-F showed higher anti-<i>helicobacter pylori</i> activity (MIC = 3.9 µg/mL) than clarithromycin (MIC = 1.95 µg/mL). The DCM-F inhibited Herpes Simplex Virus (HSV) Type I (IC₅₀ = 29.85 µg/mL) and HSV Type II (IC₅₀ = 74.17 µg/mL). The Bu-F showed higher anti-mycobacterial activity (MIC = 7.81 µg/mL) than isoniazid (MIC = 0.24 µg/mL) and higher antibacterial activity against <i>methicillin-resistant Staphylococcus aureus</i> (MRSA)(MIC = 7.81 µg/mL) than vancomycin (MIC = 3.9 µg/mL). Antioxidant assays included total antioxidant capacity (TAC), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and iron reducing power. The Bu-F showed the highest antioxidant activity. Chemical profiles were analyzed using HPLC-HR-ESI-MS to identify the metabolites responsible for these biological activities. We identified more than 60 metabolites that belong to anthocyanins, flavonoids, phenolics, terpenes, sterols, and fatty acids. In conclusion, <i>Hibiscus</i><i>schizopetalus</i> is endowed with metabolites that could be used against viruses and antibiotic-resistant bacteria. They can also be potent antioxidants.