Succinate-treated macrophages attenuate dextran sodium sulfate colitis in mice

Abstract

rophages and can affect disease development such as IBD. Wang et al. Hence, to identify the direct effect of succinate on macrophages, we performed a transfer experiment to find out the effects of succinate on macrophages in a dextran sulfate sodium (DSS) (MP Biomedicals, Solon, OH, USA)-induced colitis model (Fig. All experiments using animals were reviewed and approved by the Institutional Animal Care and Use Committee of Yonsei University Severance Hospital (IA-CUC No: 2018-0227), and all methods were performed in accordance with relevant guidelines and regulations. Eight-weekold male C57BL/6 mice were maintained in 12 hours light/12 hours dark cycle at 22C under specific pathogen-free conditions and used as donors of peritoneal macrophages. The following groups of mice were used as donors: injected vehicle (Veh) (n = 4); injected succinate (Suc) (n = 4). F4/80 + peritoneal macrophages were isolated using BD FACS Aria II (BD Biosciences, San Jose, CA, USA) from the collected peritoneal cells of mice which were then intraperitoneally injected with 40 mM succinate or not for 3 days (Fig. Isolated F4/80 + cells of 2 10 5 were intraperitoneally injected to recipient mice in 200 L Hanks' balanced salt solution (HBSS; Thermo Fisher Scientific, Inc., Waltham, MA, USA) (day 0) and the mice were sacrificed after 2% (wt/vol) DSS in drinking water until day 6 followed by normal water administrated for 2 days.