The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms

Abstract

The research has been carried out with a focus on the assessment of the antimicrobial efficacy of pure nanohydroxyapatite, Cu²⁺-doped nanohydroxyapatite, ozonated olive oil-loaded nanohydroxyapatite, and Cu²⁺-doped nanohydroxyapatite, respectively. Their potential antimicrobial activity was investigated against <i>Streptococcus mutans, Lactobacillus rhamnosus</i>, and <i>Candida albicans</i>. Among all tested materials, the highest efficacy was observed in terms of ozonated olive oil. The studies were performed using an Ultraviolet-Visible spectrophotometry (UV-Vis), electron microscopy, and statistical methods, by determining the value of Colony-Forming Units (CFU/mL) and Minimal Inhibitory Concentration (MIC).