Use of Temperature-Sensitive Liposomes in the Selective Delivery of Methotrexate and Cis-Platinum Analogues to Murine Bladder Tumor

Abstract

This report describes the release of methotrexate and cis-platinum analogues JM8 and JM9 from phase transition liposomes. Large unilamellar liposomes were able to quickly release the antineoplastic agents employed during in vitro tests. Methotrexate exhibited a 73 per cent release in one second. The cis-platinum analogues JM8 and JM9 were slower, showing 43.5 per cent and 33.5 per cent release at two seconds. In vivo experiments utilizing MBT-2 transitional cell carcinoma-bearing mice were conducted. Methotrexate delivered by the liposome complex showed an 8.4-fold increase in heated tumor uptake when compared to unheated tumors.