Engineering Inorganic Nanoflares with Elaborate Enzymatic Specificity and Efficiency for Versatile Biofilm Eradication

Abstract

Nanozyme has emerged as a versatile nanocatalyst yet is constrained with limited catalytic efficiency and specificity for various biomedical applications. Herein, by elaborately integrating the recognition/transduction carbon dots (CDs) with platinum nanoparticles (PtNPs), an exquisite CDs@PtNPs (CPP) nanoflare is engineered as an efficient and substrate-specific peroxidase-mimicking nanozyme for high-performance biosensing and antibacterial applications. The intelligent CPP-catalyzed hydrogen peroxide (H₂ O₂ )-generated reactive oxygen species realize the sensitive diagnosis-guided enhanced disinfection of pathogens. Significantly, the CPP nanozyme shows the prominent biofilm eradication and wound healing in vivo by virtue of endogenous H₂ O₂ in acidic infection tissues, which can substantially preclude the annoying antibiotics resistance. A fundamental understanding on the present CPP nanoflare would not only facilitate the advancement of various prospective biocatalysts, but also establish a multifunctional means for versatile biosensing and smart diagnostic applications.