Oxidative Medicine and Cellular Longevity · 2020 · 33 citations · 40 references
Life SciencesBiochemistryPhysiologyMetabolic PhysiologyMetabolic RegulationStress PhysiologyDiabetes MellitusInsulin ResistanceMetabolic SyndromeWeight ManagementGlutathione S-transferase-mediated MetabolismMetabolic Consequences Of StressMetabolic Syndrome AssociationsNitric OxideNitrosative DamageObesity-related Stress ResponsesOxidative Stress In Metabolic DisordersStress
In this pathbreaking study, we evaluated nitrosative stress in morbidly obese patients with and without metabolic syndrome. 62 women with class 3 obesity (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mtext>BMI</mml:mtext><mml:mo>></mml:mo><mml:mn>40</mml:mn><mml:mtext> </mml:mtext><mml:mtext>kg</mml:mtext><mml:mo>/</mml:mo><mml:msup><mml:mrow><mml:mtext>m</mml:mtext></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup></mml:math>) were divided into three subgroups: obese patients (OB), obese patients with hypertension (OB+HYP), and obese patients with metabolic syndrome (OB+MS). In comparison to the lean patients, OB had increased levels of serum myeloperoxidase (MPO), plasma nitric oxide (NO), S-nitrosothiols, and peroxynitrite (ONOO − ), as well as nitrotyrosine, while oxidized glutathione (GSSG) rose only in OB+HYP group. Interestingly, ONOO − was significantly higher in OB+HYP and OB+MS as compared to OB group, while MPO only in OB+MS group. OB+MS had greater nitrotyrosine and S-nitrosothiol values than OB+HYP. Moreover, peroxynitrite could differentiate OB from OB+HYP and OB+MS (AUC 0.9292; <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>p</mml:mi><mml:mo><</mml:mo><mml:mn>0.0001</mml:mn></mml:math>; 87.5% sensitivity, 90% specificity) as well as between OB and OB+MS group (AUC 0.9125; <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mi>p</mml:mi><mml:mo><</mml:mo><mml:mn>0.0001</mml:mn></mml:math>; 81.25% sensitivity, 83.33%). In conclusion, we showed that MPO activity, NO formation, and nitrosative damage to proteins parallel the progression of metabolic disturbances of obesity. Evaluation of ONOO − concentrations may help predict the development of hypertension and metabolic syndrome in patients with morbid obesity; however, longer-term studies are required for larger numbers of patients.
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