Two new diterpenoids from<i>Penicillium chrysogenum</i>MT-12, an endophytic fungus isolated from<i>Huperzia serrata</i>

Abstract

Two new diterpenoids, penicichrysogene A (<b>1</b>) and penicichrysogene B (<b>2</b>), were isolated from the solid substrate fermentation cultures of <i>Penicillium chrysogenum</i> MT-12, an endophytic fungus isolated from the medicinal plant of <i>Huperzia serrata</i>. Their structures were elucidated on the basis of extensive spectroscopic and spectrometric data (1D and 2D NMR, UV, IR, and HRESIMS). The absolute configurations of <b>1</b> and <b>2</b> were assigned on the basis of experimental and calculated electronic circular dichroism spectra. Compound <b>1</b> exhibited inhibitory activity on ATP release of thrombin-activated platelets with IC₅₀ = 42.7 ± 3.5 μM.