Skermanella pratensis sp. nov., isolated from meadow soil, and emended description of the genus Skermanella

Abstract

A novel Gram-stain-negative, light pink-coloured, short rod-shaped, designated strain W17^T, was isolated from a meadow soil sample collected from Xinjiang, PR China. The 16S rRNA gene sequence analysis indicated that strain W17^T was related most closely to <i>Skermanella rosea</i> M1^T (98.72 %) and <i>Skermanella mucosa</i> 8-14-6^T (98.44 %). However, strain W17^T showed a low level of DNA-DNA relatedness to <i>S. rosea</i> M1^T (32.4±2.6 %) and <i>S. mucosa</i> 8-14-6^T (33.5±0.1 %). The genome size of the novel strain was 5.87 Mb and the genomic DNA G+C content was 67.27 mol%. The only respiratory quinone of strain W17^T was Q-10. Diphosphatidylglycerol, phosphatidylglycerol. phosphatidylethanolamine and phosphatidylcholine were the major polar lipids. The predominant cellular fatty acids were C_{18 : 1}ω6<i>c</i> and/or C_{18 : 1}ω7<i>c</i> (48.53 %), C_{16 : 0} (20.88 %) and C_{18 : 0} (14.92 %). The phylogenetic, phenotypic and chemotaxonomic data showed that strain W17^T represents a novel species of the genus <i>Skermanella</i>, for which the name <i>Skermanella pratensis</i> sp. nov. is proposed. The type strain is W17^T (=GDMCC 1.1392^T=KCTC 62434^T).