Diversity in the Extracellular Vesicle-Derived Microbiome of Tissues According to Tumor Progression in Pancreatic Cancer

Abstract

This study was conducted to identify the composition and diversity of the microbiome in tissues of pancreatic cancer and to determine its role. First, extracellular vesicles (EVs) were obtained from the paired tumor and normal tissues, and 16s rRNA gene sequencing was performed. We identified the microbiomes, compared the diversity between groups, and found that <i>Tepidimonas</i> was more abundant in tumors. Second, larger tumors resulted in lower levels of <i>Leuconostoc</i> and <i>Sutterella</i>, and increased lymph node metastasis resulted in higher levels of <i>Comamonas</i> and <i>Turicibacter</i> in tumor tissues. Moreover, in the case of tumor recurrence, the levels of <i>Streptococcus</i> and <i>Akkermansia</i> were decreased in tumor tissues. Finally, with the supernatant of <i>Tepidimonas</i><i>fonticaldi</i>, proliferation and migration of cells increased, and epithelial-mesenchymal transition and the Tricarboxylic Acid (TCA) cycle-related metabolites were enhanced. The composition and diversity of EV-derived microbiomes are important for providing novel insights into theragnostic approaches in pancreatic cancer.