Toxicity Assessment of SiO2 and TiO2 in Normal Colon Cells, In Vivo and in Human Colon Organoids

Abstract

We conducted systemic assessments on the toxicity of silicon dioxide (SiO₂) and titanium dioxide (TiO₂) nanoparticles using different forms of normal colon cells (CCD-18Co), in vivo and in human colon organoids. The in vivo acute oral toxicity data showed that the LD₅₀ values are greater than 2000 mg/kg for both the SiO₂ and TiO₂ nanoparticles; however, the SiO₂ and TiO₂ nanoparticles induced cytotoxicity in two-dimensional CCD-18Co cells and three-dimensional CCD-18Co spheroids and human colon organoids, with IC₅₀ values of 0.6, 0.8 and 0.3 mM for SiO₂ and 2.5, 1.1 and 12.5 mM for TiO₂ nanoparticles, respectively. The data suggest that, when SiO₂ and TiO₂ are in nanoparticle form, cytotoxicity is induced; thus, care should be taken with these materials.