Combined high leaf hydraulic safety and efficiency provides drought tolerance in <i>Caragana</i> species adapted to low mean annual precipitation

Abstract

Clarifying the coordination of leaf hydraulic traits with gas exchange across closely-related species adapted to varying rainfall can provide insights into plant habitat distribution and drought adaptation. The leaf hydraulic conductance (K_leaf ), stomatal conductance (g_s ), net assimilation (A), vein embolism and abscisic acid (ABA) concentration during dehydration were quantified, as well as pressure-volume curve traits and vein anatomy in 10 Caragana species adapted to a range of mean annual precipitation (MAP) conditions and growing in a common garden. We found a positive correlation between Ψ_leaf at 50% loss of K_leaf (K_leaf P₅₀ ) and maximum K_leaf (K_leaf-max ) across species. Species from low-MAP environments exhibited more negative K_leaf P₅₀ and turgor loss point, and higher K_leaf-max and leaf-specific capacity at full turgor, along with higher vein density and midrib xylem per leaf area, and a higher ratio of K_leaf-max : maximum g_s . Tighter stomatal control mediated by higher ABA accumulation during dehydration in these species resulted in an increase in hydraulic safety and intrinsic water use efficiency (WUE_i ) during drought. Our results suggest that high hydraulic safety and efficiency combined with greater stomatal sensitivity triggered by ABA production and leading to greater WUE_i provides drought tolerance in Caragana species adapted to low-MAP environments.