Concepedia

Assessment of human plasma and urine sample preparation for reproducible and high-throughput UHPLC-MS clinical metabolic phenotyping

Andrew D. Southam, Liam D. Haglington, Lukáš Najdekr, Andris Jankevics, Ralf J. M. Weber, Warwick B. Dunn

The Analyst · 2020 · 53 citations · 38 references

DOIFull text

Open access

Concepts

Abstract

UHPLC-MS plasma lipidomics, monophasic 100% isopropanol had the highest detection response of all annotated lipid classes with high reproducibility. Increasing monophasic incubation time and temperature had little benefit on metabolite and lipid yield and reproducibility for all methods.

References

38

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