Analytical Chemistry · 2020 · 113 citations · 35 references
Natural SciencesCell BiologyChemistryAnalytical ChemistryBiophotonicsMolecular ImagingFluorescence ImagingFluorescence SpectroscopyOptical BiosensingOptical SpectroscopyPhotochemistryPhotophysics Of Fluorescent ProbesChromophore DesignFluorescent Probe DesignFluorescent ProbesIntramolecular Electron TransferRatiometric Fluorescence ImagingRational DesignSelectivityStokes Shift
By introducing the tetrahydroquinoxaline group as the electron donor to enhance intramolecular charge transfer effect, we deliberately designed a coumarin derivative, TQC-HClO, to serve as a two-photon ratiometric fluorescent probe for imaging hypochlorous acid in cells and zebra fish with good sensitivity and high selectivity. TQC-HClO displayed an obvious two-photon absorbance cross-section (over 100 GM in 800–840 nm), large Stokes shifts (159 to 173 nm), and a ratiometric fluorescence change from orange (λmaxem = 583 nm) to red (λmaxem = 650 nm) in response to hypochlorous acid.
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