Amplified Electrochemical Hydrogen Peroxide Sensing Based on Cu-Porphyrin Metal–Organic Framework Nanofilm and G-Quadruplex-Hemin DNAzyme

Abstract

A novel electrochemical hydrogen peroxide (H₂O₂) sensor based on Cu-porphyrin(Cu-TCPP)/G-quadruplex-hemin nanocomposite was constructed by assembling two-dimensional Cu-TCPP metal-organic framework (MOF) nanofilm and G-quadruplex-hemin DNAzyme. The Cu-TCPP synthesized by the surfactant-assisted method has a wrinkled two-dimensional nanofilm morphology, which gives it a large surface area and accessible active sites. Cu-TCPP exhibits peroxidase activity and good stability and can catalyze the reduction of H₂O₂. In addition, Cu-TCPP can be used as a nanocarrier for G-quadruplex-hemin DNAzyme with strong peroxidase activity to achieve "biological barcode" amplification and improve stability. The cooperative interaction of Cu-TCPP and G-quadruplex-hemin DNAzyme effectively amplifies the electrochemical response signal. Electrochemical studies have shown that the constructed sensor exhibits good electrochemical sensing performance with three linear ranges: 0.08 μM to 0.11 mM, 0.11-0.91 mM, and 0.91-8.1 mM, with sensitivities of 2315.86, 301.00, and 65.71 μA/(mM cm²), respectively, and the detection limit was 0.03 μM. In addition, the sensor shows good selectivity. In summary, this study provides a simple and effective new strategy for electrochemical sensing based on two-dimensional MOFs and artificial enzymes.