Lifetime-based nanothermometry <i>in vivo</i> with ultra-long-lived luminescence

Abstract

We developed a luminescence lifetime-based nanothermometer with a single-exponential luminescence decay in the ∼s time scope via a photochemical reaction. The luminescence lifetime imaging in vivo can be performed on an EMCCD and consumer-grade camera, which significantly reduces the technical threshold of luminescence lifetime imaging.