Prevotella hominis sp. nov., isolated from human faeces

Abstract

A strictly anaerobic predominant bacterium, designated as strain gm001^T, was isolated from a freshly voided faecal sample collected from a healthy Taiwanese adult. Cells were Gram-stain-negative rods, non-motile and non-spore-forming. Strain gm001^T was identified as a member of the genus <i>Prevotella</i>, and a comparison of 16S rRNA and <i>hsp60</i> gene sequences revealed sequence similarities of 98.5 and 93.3 %, respectively, demonstrating that it was most closely related to the type strain of <i>Prevotella copri</i>. Phylogenomic tree analysis indicated that the gm001^T cluster is an independent lineage of <i>P. copri</i> DSM 18205^T. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between strain gm001^T and <i>P. copri</i> DSM 18205^T were 80.9, 28.6 and 83.8 %, respectively, which were clearly lower than the species delineation thresholds. The species-specific genes of this novel species were also identified on the basis of pan-genomic analysis. The predominant menaquinones were MK-11 and MK-12, and the predominant fatty acids were anteiso-C_{15 : 0}, C_{15 : 0} and iso-C_{15 : 0}. Acetate and succinate were produced from glucose as metabolic end products. Taken together, the results indicate that strain gm001^T represents a novel species of the genus <i>Prevotella</i>, for which the name <i>Prevotella hominis</i> sp. nov. is proposed. The type strain is gm001^T (=BCRC 81118^T=JCM 33280^T).