Circular RNA Expression Profile and m6A Modification Analysis in Poorly Differentiated Adenocarcinoma of the Stomach

Abstract

<b>Aims:</b> To profile and characterize the circular RNA (circRNA) expression pattern in poorly differentiated gastric adenocarcinoma (PDGA). <b>Methods & materials:</b> CircRNA expression profiles in PDGA and adjacent nontumor tissues were analyzed by microarray. Five randomly selected differentiated expressed circRNAs (DECs) were validated by real-time quantitative PCR. m6A qualification of the top 20 DECs was conducted by m6A-immunoprecipitation and real-time quantitative PCR. <b>Results:</b> A total of 65 DECs were found in PDGA compared with the control. Hsa_circRNA_0077837 had the largest area under the curve. Most DECs had m6A modifications, the trend of m6A modification alteration was mainly consistent with the circRNA expression level. <b>Conclusion:</b> Our study revealed a set of DECs and their m6A modification alterations, which may provide new insight for their potential function in PDGA.