Science · 2020 · 1.4K citations · 48 references
Life SciencesInfectious DiseasesMolecular BiologyMolecular VirologyStructural BiologyVirologyProtein StructureViral MaturationViral Protein ProcessingViral StructureVirus EntryVirus StructureConformational Transition StatesSars-cov-2 InfectionViral Envelope GlycoproteinsViral Envelope ProteinsViral Envelope StructureViral Protein ConformationViral Protein FoldingViral Spike Proteins
The SARS‑CoV‑2 spike (S) protein is a dynamic trimer that mediates viral entry into host cells. The study aims to elucidate the structural transitions of the S protein that drive fusion of viral and host membranes. Full‑length S protein was purified and cryo‑electron microscopy was used to resolve both prefusion and postfusion conformations. The resulting structures reveal a stabilized prefusion ectodomain and provide insights into S protein function that may guide vaccine design. Cai et al., Science, p.
A dynamic viral spike Efforts to protect human cells against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have focused on the trimeric spike (S) protein. Several structures have shown a stabilized ectodomain of the spike in its prefusion conformation. Cai et al. now provide insight into the structural changes in the S protein that result in the fusion of the viral and host cell membranes. They purified full-length S protein and determined cryo–electron microscopy structures of both the prefusion and postfusion conformations. These structures add to our understanding of S protein function and could inform vaccine design. Science , this issue p. 1586
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