The electric potential distribution profile in a naturally charged fluidized bed and its effects

Abstract

The authors describe an amplified photoelectrochemical immunoassay for the tumor marker carbohydrate antigen 724 (CA724). The method employs a C₃N₄-MoS₂ semiconductor as the photoelectric conversion layer. The nanocomposite was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction, and UV-vis diffuse reflectometry. The dye eosin Y was encapsulated into CaCO₃ nanospheres which then were used as labels for antibody against CA724. In addition, Fe₃O₄ nanospheres were employed as magnetic platform for constructing photoelectrochemical sandwich immunoassay. The CaCO₃ nanospheres can be dissolved with aid of ethylene diamine tetraacetic acid (EDTA) and the carried eosin Y in CaCO₃ is released. The released dyes sensitizes the C₃N₄-MoS₂ semiconductor, which induces photocurrent amplification. Under optimal conditions and at a typical working voltage of 0 V (vs. SCE), the photocurrent increases linearly in the range of 0.05 mU mL^-1 to 500 mU mL^-1 of CA724, with a 0.02 mU mL^-1 detection limit. Graphical abstract The C₃N₄-MoS₂ complex, with high efficiency of electron transport, was synthesized to construct a photoelectrochemical analytical platform. A sandwich-type immunoassay was established on the surface of magnetic beads. Carbohydrate antigen 724 in sample was detected sensitively by using sensitization of released eosin Y as signal amplifiery.