Physicochemical characterization and antimicrobial properties of Mahamanjishthadi kadha: An Ayurvedic formulation

Abstract

Skin infections are common throughout the world. With high infectivity amongst skin pathogens, approximately 300 million people are affected worldwide. The increasing rate of failure of chemotherapeutics and antibiotic resistance exhibited by pathogenic microbial infectious agents, has increased the use of Ayurvedic medicines. This study aims to standardize an Ayurvedic formulation, Mahamanjisthadi kadha and to evaluate its antimicrobial properties against skin infection, causing pathogens. Physicochemical analysis such as organoleptic tests, pH, alcohol content, Brix and Specific gravity was done. Phytochemical screening was performed for various bioactive compounds. Heavy metals, aflatoxins and microbial load were checked for contaminants. Chromatographic analysis was performed to estimate lupeol, ellagic acid and gallic acid, using high performance thin layer chromatography (HPTLC). Antimicrobial activity was determined against five common pathogens causing skin infections, using well-diffusion method.