Characterization of chemical components with diuretic potential from <i>Pyrrosia petiolosa</i>

Abstract

Three compounds with diuretic potential were identified from the 95% ethanol extract of <i>Pyrrosia petiolosa</i> (Christ) Ching. Among them, one was a new benzanilide named petiolide A (<b>1</b>), and the other two were phenolic derivatives barbatic acid (<b>2</b>) and kaempferol (<b>3</b>). Their structures were elucidated based on extensive spectral analyses and comparison with the literature data. The docking experiments of all compounds into the active site of the With-No-Lysine kinase 1 (WNK1) domain demonstrated that kaempferol (<b>3</b>) was the most effective component with diuretic potential for its comparative diuretic effect to that of an orally bioavailable WNK inhibitor WNK463 (docking score -10.99 vs -11.09).[Formula: see text].