Transcriptome alterations and genotoxic influences in zebrafish larvae after exposure to dissolved aluminum and aluminum oxide nanoparticles

Abstract

Manufactured nanoparticles (NPs) can potentially cause negative effects on molecular (proteins and nucleic acids), subcellular, cellular, tissue, organ, and organism due to their unusual physicochemical characteristics. Ionizable NPs in water (e.g., Al₂O₃-NPs) may create toxic effects on aquatic animals. The present research determined the influences of Al₂O₃-NPs and appropriate concentrations of ionizing Al(III) using water-soluble AlCl₃ in zebrafish larvae (72 h post-fertilization, <i>Danio rerio</i>) by analyzing transcriptional alterations of stress-associated genes (<i>rad51</i>, <i>p53</i>, <i>mt2</i>) with quantitative real-time PCR (qRT-PCR). In addition, genotoxic effects of Al(III) and Al₂O₃-NPs were evaluated. The lethal concentrations that cause death of 50% (LC₅₀) of zebrafish larvae when exposed to 0-50 mg/l Al(III) and 0-500 mg/l Al₂O₃-NPs were 3.26 ± 0.38 and 130.19 ± 5.59 mg/l, respectively, for 96 h. A concentration-dependent increase was observed in the genotoxicity in cells of larvae exposed to Al(III) and Al₂O₃-NPs for 96 h. DNA damage was more severe in larvae exposed to Al(III) (41.0% tail) than that of Al₂O₃-NPs (21.8% tail). A complex induction of stress-associated genes was observed in fish and this induction was not directly related to the concentrations of Al(III) and Al₂O₃-NPs, although a significant induction was detected in <i>mt2</i> gene of larvae exposed to Al(III) and Al₂O₃-NPs relative to control group. The induction levels of <i>mt2</i> were 4.13 ± 0.1 and 2.13 ± 0.1-fold change (mean ± S.E.M.) in larvae at 15 mg/l of Al(III) and 100 mg/l of Al₂O₃-NP concentrations, respectively.