INVESTIGATION OF PHYTOCHEMICAL CONSTITUENTS AND CARDIOPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF BEETROOT (Beta vulgaris. L) ON DOXORUBICIN INDUCED TOXICITY IN RAT

Abstract

Doxorubicin is used extensively to treat cancer. Oxidative stress condition is closely related to doxorubicin therapy, which is characterized by the formation of lipid peroxidation which has the potential to cause cell damage and induced cardiotoxicity. This study was to determine the cardioprotective effect of beetroot ethanol extract on a rat induced by doxorubicin. As many as 30 Male white rats as weighing 180-200 grams were divided into six treatment groups. Group, I was the normal control group without treatment. Group II was a negative control group that only injected doxorubicin with a cumulative dose of 15 mg/kgBW for 15 days. Group III was the positive control group that treated by vitamin E 100 mg/kgBW, Group IV-VI was the test group that treated by beetroot ethanol extract at doses of 100, 200 and 400 mg/kgBW respectively for 15 days together with doxorubicin induction. The group treated with beetroot extract at a dose of 100 mg/kgBW could improve the condition of rat in all parameters including Creatine kinase-MB (CK-MB) level, Lactate Dehydrogenase (LDH) level and hematology profile and it was significantly different to the negative control (p <0.05). All parameters showed that the beetroot extract had a cardioprotective and it showed a dose-dependent manner. This research proves that the compound contained in beetroot ethanol extract has protective activity in rats that induced by doxorubicin.