Carbon‐dot‐based ratiometric fluorescent probe of intracellular zinc ion and persulfate ion with low dark toxicity

Abstract

Metal ions and anions play significant roles in biological systems and industrial processes, therefore it is important to develop good fluorescent probes to detect metal ions and anions. Here, N,O-co-doped carbon quantum dots (CDs) that could detect Zn²⁺ via a ratiometric fluorescence method were fabricated. The reaction between the as-prepared CDs and zinc acetate gave the composite CDs-Zn, in which fluorescence changed ratiometrically upon addition of S₂ O₈ ^2- . With change in excitation light, the emission peaks of the CDs and CDs-Zn were kept fixed while intensity changed. CDs and CDs-Zn exhibited good photostability, thermal stability, selectivity, and strong anti-interference ability. In addition, CDs and CDs-Zn displayed low dark toxicity under physiological temperatures. Ratiometric fluorescence imaging of intracellular Zn²⁺ and S₂ O₈ ^2- was carried out in living HeLa cells for both of these probes. Compared with reported ratiometric fluorescent hybrid nanosensors based on organic dyes and inorganic nanomaterials, the as-prepared CDs and CDs-Zn had low toxicity, and were easy to synthesize.