Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells

TLDR

Organoids are 3D constructs that mimic tissue development but have historically lacked stromal components such as blood vessels. The study presents a protocol to incorporate stromal elements, particularly vasculature, by co‑culturing organoids with iPSC‑derived mesodermal progenitor cells. Detailed protocol execution is provided in Wörsdörfer et al. Published in 2019.

Abstract

Organoids are three-dimensional (3D) constructs generated in stem cell cultures and are thought to mimic tissue and organ development in situ. However, until recently, they often exclusively recapitulated the development of the organ`s parenchyma without the major components of the organ stroma. Here, we describe a protocol to incorporate stromal components, first of all blood vessels, by co-culturing with induced pluripotent stem cell-derived mesodermal progenitor cells. For complete details on the use and execution of this protocol, please refer to Wörsdörfer et al. (2019).