Identification of enzymatic genes with the potential to reduce biomass recalcitrance through lignin manipulation in Arabidopsis

Abstract

Simple colorimetric characterization of stem lignin is useful for simultaneous screening of many genes with the potential to reduce lignin recalcitrance. In addition to <i>F5H</i>, the positive control, we identified three enzyme-coding genes that can function as genetic tools for lignin manipulation. Two of these genes (<i>calB</i> and <i>couA</i>) accelerate sugar release from transgenic lignocelluloses.