Fluispotter, a Novel Automated and Wearable Device for Accurate Volume Serial Dried Blood Spot Sampling

Abstract

<b>Aim:</b> A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. <b>Materials & methods:</b> An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. <b>Results:</b> The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. <b>Conclusion:</b> The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.