Construction of gold-siRNA_NPR1 nanoparticles for effective and quick silencing of <i>NPR1</i> in <i>Arabidopsis thaliana</i>

Abstract

In recent years, gold nanoparticles (AuNPs) have been widely used as gene silencing agents and therapeutics for treatment of cancers due to their high transfection efficiency and lack of cytotoxicity, but their roles in gene silencing in plants have not yet been reported. Here, we report synthesis of AuNPs-branched polyethylenimine and its integration with the small interfering RNAs (siRNA) of <i>NPR1</i> to form a AuNPs-siRNA _<i>NPR1</i> compound. Our results showed that AuNPs-siRNA _<i>NPR1</i> was capable of infiltrating into <i>Arabidopsis</i> cells. AuNPs-siRNA _<i>NPR1</i> silenced 80% of the <i>NPR1</i> gene in <i>Arabidopsis</i>. Bacteriostatic and ion leakage experiments suggest that the <i>NPR1</i> gene in <i>Arabidopsis</i> leaves was silenced by AuNPs-siRNA _<i>NPR1</i> . In Columbia-0 plants, compared with the control group treated with buffer solution, the AuNPs-siRNA _<i>NPR1</i> treatment significantly increased the number of colonies and cell death, and the leaves turned yellow, similar to the phenotype of the <i>npr1</i> leaves. These results indicated this AuNPs-siRNA _<i>NPR1</i> silencing the <i>NPR1</i> gene method is simple, effective and quick (3 days), and a powerful tool to study gene functions in plants.